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thrombin plus convulxin  (Thermo Fisher)


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  • 97

    Structured Review

    Thermo Fisher thrombin plus convulxin
    Thrombin Plus Convulxin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thrombin+plus+convulxin/THROMBIN/pm24632563-338-9-71
    Average 97 stars, based on 1 article reviews
    thrombin plus convulxin - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Platelet production proceeds independently of the intrinsic and extrinsic apoptosis pathways.
    Article Snippet: .. To determine platelet exposure of phosphatidylserine in response to thrombin plus convulxin (Thr 0.5–1 U ml 1; Cvx 50–100 ng ml 1, 10 min RT), ionophore A23187 (1mM, 10 min), cross-linked FasL (2mg ml 1, 2 h at 37 C) or the intrinsic apoptosis inducer ABT-737 (0.06–0.5 mM, 90 min 37 C), treated platelets were incubated for 20 min at RT with Annexin V and CD41-APC mAb in Annexin V buffer (Invitrogen) before flow cytometric analysis. ..



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    Thermo Fisher thrombin plus convulxin
    Thrombin Plus Convulxin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology thrombin plus convulxin
    (A) Representative density plots showing P-selectin surface expression, Δ(X00471)m and phosphatydilserine (PhSer) exposure in platelets activated with thrombin (Thr) and/or <t>convulxin</t> (Cvx). (B-F) Monocytes were exposed to resting platelets or platelets activated with Thr or Thr+Cvx in the presence or absence of neutralizing antibodies against CD62-P or PhSer. The concentrations of IL-8 (B, C and E) and IL-10 (B, D and F) in the supernatants of cells incubated in each condition are shown. The bars represent mean±SEM of 4-8 independent experiments. The asterisk (*) signifies p<0.05 compared to unstimulated platelets. The pound sign (#) indicates p<0.05 compared to IgG.
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    Image Search Results


    (A) Representative density plots showing P-selectin surface expression, Δ(X00471)m and phosphatydilserine (PhSer) exposure in platelets activated with thrombin (Thr) and/or convulxin (Cvx). (B-F) Monocytes were exposed to resting platelets or platelets activated with Thr or Thr+Cvx in the presence or absence of neutralizing antibodies against CD62-P or PhSer. The concentrations of IL-8 (B, C and E) and IL-10 (B, D and F) in the supernatants of cells incubated in each condition are shown. The bars represent mean±SEM of 4-8 independent experiments. The asterisk (*) signifies p<0.05 compared to unstimulated platelets. The pound sign (#) indicates p<0.05 compared to IgG.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Platelet activation and apoptosis modulate monocyte inflammatory responses in dengue

    doi: 10.4049/jimmunol.1400091

    Figure Lengend Snippet: (A) Representative density plots showing P-selectin surface expression, Δ(X00471)m and phosphatydilserine (PhSer) exposure in platelets activated with thrombin (Thr) and/or convulxin (Cvx). (B-F) Monocytes were exposed to resting platelets or platelets activated with Thr or Thr+Cvx in the presence or absence of neutralizing antibodies against CD62-P or PhSer. The concentrations of IL-8 (B, C and E) and IL-10 (B, D and F) in the supernatants of cells incubated in each condition are shown. The bars represent mean±SEM of 4-8 independent experiments. The asterisk (*) signifies p<0.05 compared to unstimulated platelets. The pound sign (#) indicates p<0.05 compared to IgG.

    Article Snippet: Each experimental point contained 5×10 5 monocytes and 5×10 7 platelets in a volume of 200 μL of M199 containing 10μg/mL of polymyxin B. Platelets were stimulated with thrombin (Sigma-Aldrich, T1063) (0.5 U/mL) or thrombin plus convulxin (Santa Cruz, sc-202554) (250 ng/mL) for 5 min.

    Techniques: Expressing, Incubation

    (A-B) Representative density plots (A) and bar graphs (B) showing the percent of platelet phagocytosis by monocytes exposed to resting platelets, or platelets stimulated with thrombin or thrombin plus convulxin in the presence or absence of anti-CD62P, anti-phosphatidylserine (PhSer) or cytochalasin D plus cytochalasin B (CytoD+B). (C-D) The concentrations of IL-8 (C) and IL-10 (D) in the supernatants of cells treated with CytoD+B are shown. (E-F) Monocyte adhesion on plates coated with human serum albumin (HSA), P-selectin (CD62P), PhSer, or P-selectin + PhSer. (G) The concentrations of IL-8 and IL-10 in the supernatants of monocytes adherent to each substrate are shown. Panels A and E show representative images and density plots from 4 independent experiments. The values in each region of the quadrants indicate the cell frequencies of the quadrant. The bars represent mean±SEM of 4 independent experiments. The asterisk (*) signifies p<0.05 compared to unstimulated platelets or HAS-coated plates. The pound sign (#) indicates p<0.05 compared to IgG or vehicle (DMSO).

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Platelet activation and apoptosis modulate monocyte inflammatory responses in dengue

    doi: 10.4049/jimmunol.1400091

    Figure Lengend Snippet: (A-B) Representative density plots (A) and bar graphs (B) showing the percent of platelet phagocytosis by monocytes exposed to resting platelets, or platelets stimulated with thrombin or thrombin plus convulxin in the presence or absence of anti-CD62P, anti-phosphatidylserine (PhSer) or cytochalasin D plus cytochalasin B (CytoD+B). (C-D) The concentrations of IL-8 (C) and IL-10 (D) in the supernatants of cells treated with CytoD+B are shown. (E-F) Monocyte adhesion on plates coated with human serum albumin (HSA), P-selectin (CD62P), PhSer, or P-selectin + PhSer. (G) The concentrations of IL-8 and IL-10 in the supernatants of monocytes adherent to each substrate are shown. Panels A and E show representative images and density plots from 4 independent experiments. The values in each region of the quadrants indicate the cell frequencies of the quadrant. The bars represent mean±SEM of 4 independent experiments. The asterisk (*) signifies p<0.05 compared to unstimulated platelets or HAS-coated plates. The pound sign (#) indicates p<0.05 compared to IgG or vehicle (DMSO).

    Article Snippet: Each experimental point contained 5×10 5 monocytes and 5×10 7 platelets in a volume of 200 μL of M199 containing 10μg/mL of polymyxin B. Platelets were stimulated with thrombin (Sigma-Aldrich, T1063) (0.5 U/mL) or thrombin plus convulxin (Santa Cruz, sc-202554) (250 ng/mL) for 5 min.

    Techniques: